DNA

Part:BBa_K5159012:Design

Designed by: Tianyue WU   Group: iGEM24_HKUST   (2024-09-26)


Y. lipolytica PEX10 5' homology arm


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 196
    Illegal PstI site found at 415
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 196
    Illegal PstI site found at 415
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 251
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 196
    Illegal PstI site found at 415
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 196
    Illegal PstI site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the unconventional yeast Yarrowia lipolytica shows a higher tendency of utilizing non-homologous end-joining (NHEJ) instead of HR for genomic integration, a 1000 bp homologous arm in length is necessary for the efficient knockout to occur.


Source

This part sequence is identified in the Yarrowia lipolytica genome and in our project synthesized by commercial provider GenScript.

References